|Year : 2021 | Volume
| Issue : 1 | Page : 45-50
Evaluation of anti-helmintic activity of Zingiber officinale roscoe extract on Fasciola hepatica miracidia In vitro
, Mohsen Arbabi1
, Mahdi Mosayebi2, Hossein Hooshyar1, Ali Mohammad Nickfarjam3
1 Department of Medical Parasitology, Kashan University of Medical Sciences, Kashan, Iran
2 Department of Medical Parasitology, Arak University of Medical Sciences, Arak, Iran
3 Department of Health Information Management and Technology, Faculty of Paramedicine, Kashan University of Medical Sciences, Kashan, Iran
|Date of Submission||28-Nov-2020|
|Date of Decision||03-Mar-2021|
|Date of Acceptance||24-Jan-2021|
|Date of Web Publication||31-Mar-2021|
Prof. Mohsen Arbabi
15th Khordad Square, Abazar Street, P.O. Box: 87137.81147, Kashan
Source of Support: None, Conflict of Interest: None
Aims: The control of fascioliasis has depended on the utilization of a predetermined number of anthelmintic drugs. Nonetheless, the resistance of antifasciolid is presently boundless, and there is a need for potential drug properties of medicinal plants as new medications against Fasciola spp. to interrupt the parasite transmission. Materials and Methods: This investigation is meant to assess the potential anti-fasciolicide impacts of Zingiber officinale roscoe hydroalcoholic extract against the Fasciola miracidia. The eggs of Fasciola hepatica were gathered from the livers contaminated goats and sheep and washed 20 times with chlorine-free water, and afterward incubated at different pH, temperature, and light density for embryonic development. Concentrated miracidia of Fasciola spp. were incubated at different concentrations (2, 5, and 10 μg/mL) of hydroalcoholic extract of Z. officinale at different time intervals. The data were analyzed using the ANOVA statistical test. Results: The extract at different concentrations showed antifasciolid effects in comparison to triclabendazole. This braking action was dose-proportional and further related firmly to the disclosure time ( P < 0.001). In concentration of 10ug/ml extract killed the parasites at 105 ± 3 s ( P < 0.001). Conclusion: In vitro antimiracidia activities of the hydro alcoholic extract of Z. officinale was satisfactory and potent fasciolicide effective, however, in vivo impact of this extract, remains for extra assessment. In this manner, these therapeutic plant extracts might be seen as confident origins of bioactive composites that could be matured against miracidia. This is the main work to evaluate the potential enemy of fasciolicide impact of certain plants against Fasciola miracidia.
Keywords: Anti-miracidia activity, Fasciola hepatica, hatch ability, Zingiber officinale roscoe
|How to cite this article:|
Ghafar A, Arbabi M, Mosayebi M, Hooshyar H, Nickfarjam AM. Evaluation of anti-helmintic activity of Zingiber officinale roscoe extract on Fasciola hepatica miracidia In vitro. Int Arch Health Sci 2021;8:45-50
|How to cite this URL:|
Ghafar A, Arbabi M, Mosayebi M, Hooshyar H, Nickfarjam AM. Evaluation of anti-helmintic activity of Zingiber officinale roscoe extract on Fasciola hepatica miracidia In vitro. Int Arch Health Sci [serial online] 2021 [cited 2022 Jan 20];8:45-50. Available from: http://www.iahs.kaums.ac.ir/text.asp?2021/8/1/45/312704
| Introduction|| |
The food-borne trematode infections represent a considerable gathering of neglected tropical diseases (NTDs) in over 10% of the world's population. Flukes of the biliary tract, as a more prevalent of food-borne trematodes, are pathogens are known well that occur in rural and urban tropical and subtropical areas have been a severe threat to human and animal health with major socioeconomic importance globally especially in developing countries., Fascioliasis is a major zoonotic problem belonging to foodborne trematodes in ruminants as a result of two liver flukes named Fasciola hepatica and F. gigentica that affect the liver and other associated organs. Human fascioliasis has been happening at times from different geographical areas of the world.
Fascioliasis is an NTD that influences millions of human beings, even more than 70 countries. It is a harsh threat to the health of animals in many regions of the world, lead to major monetary damages to the livestock industry in many areas because of decreased production of over US$3 billion each year.
Several factors such as resistance to anti-fascioliasis drugs, man-made environmental modifications, and climate alterations can influence the epidemiological pattern of the disease. Control of fascioliasis is imperative for human health as well as an animal at the farm. At the movement, however, the effectiveness of disease control is breaking down in most infected areas. Alternative effective strategies for fasciola control are grazing management, biological control, and use of antifasciolid medications in the response of fascioliasis or its effects. Data recommends that chemotherapy procedures to control the infection are inefficient, also normally select for resistant pedigrees of the Fasciola spp. that globally recommended. Because of high veterinary cost, limited available antiparasitic chemical compounds, drug resistance, and remain drug in milk and related toxicity, investigate on elective traditional properties herbs are justified.,,,
The medical treatment of fasciola infection is exceeding of manufacturing output of antiparasitic drugs, but occasionally with the use of medicinal plants. At present, many disadvantages and limitations in using chemical drugs such as worldwide increasing resistance of fascioliasis to the drug of choice, that is triclabendazole (TCBZ) is very concerning because it threatened human health.,
Nowadays, a lot of researchers have made many efforts to find the solution for this problem and discover alternative anthelmintic derived from herbs, which has its benefit that is the stable characteristic of a natural active chemical compound the diversity which can preclude the anthelmintic resistance. A review of the most effective medicinal plants for fascioliasis in traditional medicine has been considered in recent years. There are many herbs that have shown favorable anti- Fasciola spp . activities . The promising effects of Lantana camara, Allium sativum, Bocconia frutescens, Lawsonia inermis, Cajanus cajan, Opuntia ficus, Artemisia mexicana, and Piper auritum,, and mushroom such as Ganoderma applanatum and Cantharellus cibarius are recognized for their anti-fascioliasis effectiveness and inhibition miracicidal motility. Zingiber officinale Roscoe is an important the tropical valued medicinal plant that is characterized by its adaptable medical activities like hepatoprotective activities, anti-inflammatory, and antibacterial. Original research based on in vivo and in vitro assay revealed that Z. officinale and it,s competent to wield has an important nematocidal activity such as against, Angiostrongylus cantonensis, Aniskis simplex, and Ascaridia galli., Similar works showed the Cestocidal activities of Z. officinale such as effects on Hymenolepis nana and Echinococcus protoscoleces either in vivo or in vitro assay.,,
The anti-trematode activities of Z. officinale have been proven. It appeared that the Z. officinale advertised differently grade schistosomal effectiveness by decreasing eggs of S. mansoni burden and the size of hepatic granulomas in animals infected with this blood fluke. In a similar study, Z. officinale has antischistosomal activities of the blood fluke S. mansoni harbored in mice and hand over a base for successive empirical evidence as well as clinical trials. The trematode concern and the egg frequency in the stool of animals treated with Z. officinale were significantly less than in control groups. Histopathological results demonstrated a decrease in granulomatous inflammation in number and diameter in the intestine as well as the liver of treated cases in comparison to control mice. There is the theory that the mechanism of activity of Z. officinale could be peripheral and central, i.e., antihistaminic as well as an anticholinergic. Z. officinale can therefore play an important role in preventing and treating various diseases, on the other hand, it can be used as an alternative to chemical drugs.
At present, there are no traditional medicinal plant extracts and natural products accessible for the treatment and control of fascioliasis. Accordingly, this research aimed to provide the fasciolicidal effects of the extract of Zingiber officinale Roscoe against Fasciola spp. miracidia in vitro in comparison to TCBZ.
| Materials and Methods|| |
F. hepatica miracidia development (embryogenesis in eggs).
The adult fresh helminths were collected directly from the 70 infected gall bladders and livers of the sheep that were slaughtered in the abattoir of Arak City, Iran. The infected organs were transported to the research laboratory of parasitology for further assays. The removed worms were washed, with distilled water until they are separated from any attached particles for isolation and gathering the eggs.
Hatching of Fasciola eggs
For determining the effect of environmental parameters on the hatchability of Fasciola miracidia under laboratory conditions, the collected eggs were washed several times by PBS solution and centrifuged at 3000 rpm for 5 min. The supernatant was discarded and the precipitated eggs were washed three times. An amount of 100 μl of the residue containing approximately 1000 mature Fasciola eggs were considered for the next assay. The tubes containing Fasciola eggs were incubated in a dark cupboard in 10 mL of chlorine-free water at pH ranges (4–8), the temperature ranges (26°C–32°C), and intensity light ranges (840–1070) with three replicates for each examination.
Ordinary pattern hatching of Fasciola miracidia observed with an inverted microscope (×40): There were blastomeres eggs, modulated eggs, embryo evolution, and finally developed miracidium. On the 1st day of the study, it was clear cellular growth. On the 12th day, the spots were amassed in a corner of the egg. The embryonic tissue differentiation was completed and the miracidia stage of Fasciola was observed inside the egg and on the 14th day, completely miracidium was formed.
Preparation of plant extracts
The plant examined was selected based on using traditional herbal medicine. The fresh Z. officinale plant was prepared and formal taxonomic identification of the plant material used in the current research was approved by the Research Center for Nutrition in Metabolic Diseases, Kashan University of Medical Sciences.
Assessment of activity extract
Antiparasitic effects of Zingiber officinale extract
The tests were performed with the various concentrations of Z. officinale extract after complete miracidial hatching. The eggs of Fasciola were incubated for 2 weeks in suitable conditions (pH, light, and temperature) to stimulate miracidial hatching.
Then, 10 μl un-chlorinated water consist of 20 miracidia was dispensed in the wells and 50 μl of any extract at 2, 5, and 10 μg/ml concentrations were added to the wells. We used un-chlorinated water and TCBZ as negative and positive control groups. Each treatment was done in a triplicate assay for each positive and negative experimental group. The assessment of each extract against F. hepatica miracidia was analyzed under the light microscope at ×40 coupled to a digital camera in 5 min intervals (1, 5, 15, 20, and 25 min). At each evaluation time, the activity of antimiracidia of the plant extracts was measured by the comparison dead/immobile and alive miracidia with the subsequent formula.
In the present study, the time of survival (s) and speed of miracidium (mm/s) followed by hatching before and after treatment was calculated and considered as the efficiency of investigating extracts.
Calculating the speed of miracidium by specifically designed software
So far, the velocity of the Fasciola hepatica miracidium has not been precisely determined, for the first time, we designed and used specific software to evaluate the effectiveness of the medication. The software can track parasites and calculate speed, displacement, and distance.
Analysis of data
The obtained data of Fasciola miracidia were analyzed by one-way ANOVA and t-tests, followed by the Tukey posttests to determine the differences among the groups. In analyzing the data, SPSS statistical program (version 18, SPSS, IBM, Armonk, NY, USA).
| Results|| |
The anti-fasciolicide activity of Z. officinale hydroalcoholic extract was examined on time of survival and speed of Fasciola miracidia at different concentrations (2, 5, and 10 μg/ml) in comparison with positive (triclabendazole) and negative (chlorine-free water in combination with medication) control groups 1–10 min after treatment. The results showed the extract stopped the survival time as well as the speed of miracidia Fasciola. This braking action of the hydroethanolic extract was dose-proportional and further related firmly to the disclosure time ( P < 0.001). No killed parasite was shown in the negative control groups. An important positive relation was detected between the concentration of extract and fatality, time of treatment, and fatality of the miracidia ( P < 0.001). The inhibition portrait realized everywhere the trial for extract was related to that achieved with total the TCBZ in 2 ug/ml concentrations ( P < 0.001). As the concentrations of the extract increased, the survival time and the speed of miracidia decreased. A concentration of 10ug/ml Z. officinale hydroalcoholic extract killed the miracidia at 105 ± 3 s time. However, in 2ug/ml concentration this time was 520 ± 4 s [Table 1]. By increasing the concentration of the extract, the speed of the parasite was significantly reduced. Thus, at a concentration of 10ug/ml the speed of miracidia 0.08 ± 0.01 (mm/s) was determined [Table 2].
|Table 1: Comparison hydroalcoholic extract of Zingiber officinale to triclabendazole against the survival of Fasciola hepatica miracidia|
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|Table 2: Comparison hydroalcoholic extract of Zingiber officinale to triclabendazole against the speed of Fasciola hepatica miracidia|
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| Discussion|| |
The utilization of medicinal plants is getting well known because of the toxicity and side effects of modern medicines. Medicinal herbs assume a significant act in the expansion of capability therapeutic substances. Z. officinale extract has been proved to have phytochemical composing that advantageous to be anthelmintic. Phytochemical constituents that act as an anthelmintic in Z. officinale are flavonoids, saponins, tannins, terpenoids, and phenol. The present results go beyond previous reports, showing the anthelmintic activity of Z. officinale. A similar conclusion was reached by Moazeni and Khademolhoseini (2016) that demonstrated Z. officinale extract decreased the miracidia creation of F. hepatica eggs. This study showed that F. hepatica eggs are sensitive to the alcoholic extract of Z. officinale at different concentrations. The present work is the first study that investigates the impressive miracicidal activities of Z. officinale extracts, although the latter is more promising by monitoring biological parameters. The present work shows that the extract examined has a good effect against Fasciola embryonic development. This result highlights that little is known about the impact of anthelmintic treatment intervention using herbal medicine. The mechanism of this inhibitory activity is not well known and maybe the outcome of embryonic lyses utilizing the extracts' capability to penetrate the egg of the Fasciola shells.,, The entering extracts may prevent the explanation of proteins filaments and microtubules in the cytoplasm as well as cell growth during embryogenesis. However, more studies necessary to be done in explaining the mechanism function of these extracts. The obtained results can be known that Z. officinale extract has anthelmintic activity-dependent concentration and exposure time. The findings are directly in line with previous findings. Pereira et al. evaluated the possible anti-fasciolicide activity of Momordica charantia extract on the eggs of F. hepatica liver flukes in different concentrations and times. The miracicidal activities extracts of Cantharellus cibarius and Ganoderma applanatum have been reported. When in comparison to the potency of other natural origins, these extracts can be treated to have good miracicidal capabilities similar to that of albendazole, which is used antifasciolid. Consequently, these herbs can be respected as encouraging origins composites that could be evolved into a miracicide accordingly gift efficient, low cost, toxic-free, and ecologically sociable recourses to fabricate drugs for the management of fascioliasis in indigenous districts.
Recently, some investigations developed a screening assay to evaluate the impacts of herbal extracts on the development and motility of Haemonchus contortus larval stages, a very common parasite and one of the most gastrointestinal pathogenic roundworms of small ruminant livestock. It is responsible for edema, anemia, and the death of infected goats and sheep. It is shown that the extracts originated from Picria fel-terrae Lour at concentrations of 3–5 mg/ml have an extensive inhibitory effect on the development and motility of H. contortus larvae, which could be associate with the attendance of dynamic elements in these extracts. In vitro ovicidal effects of the methanolic extract of Z. officinale at different concentrations and various disclosure times on F. hepatica eggs were conducted satisfactorily.
There is little evidence on the miracidia outcome of herbal plants opposite F. hepatica is accessible. In this study, the potency of the alcoholic extract on miracidia of F. hepatica was considered. The present study showed, the Z. officinaleon extract at a concentration of 10ug/mL, after 10 min, reduced the speed and survival time miracidia of F. hepatica. Our results revealed that liver fluke F. hepatica miracidia are impressionable to the hydroalcoholic extracts of Z. officinaleon. Furthermore, chemical researches are required to separate the biological compounds accountable for miracidia activities presented in the Z. officinale. Notwithstanding, in vivo miracidia potency of these plant extracts desires additionally review moreover. According to the current study, the study extracts offer a chance for novel agents. The promising tramatodicidal effect of the extract of Z. officinale has not nevertheless been explored, and the present element most likely has some pharmacological effect contra adult F. hepatica. More investigations are going to target other phases of the life cycle of this liver fluke trematode. However, in vivo anthelmintic performance of this extract needs more studies as well. According to the results of the present work, Z. officinale offers freedom for uncommon combinations. The possible trematodicidal effectiveness of the extract of Z. officinale has not yet been surveyed, and this composite most likely has a few pharmacological activities against other stages of Fasciola spp. Our results demonstrated that Z. officinale offers an opportunity for strange compounds and casts a new light on the control of fascioliasis in endemic areas.
| Conclusion|| |
Traditional plant-based are encouraging therapeutic tools for the management of fasciola infection in humans and animals. The present work showed the promising anti-miracicidal effects of the extract of Zingiber officinale Roscoe on the embryonic development of F. hepatica eggs. The Z. officinale has a good miracicidal capability like TCBZ, which is presently used as a choice drug for the treatment of fasciolosis. Extensive knowledge on Z. officinale can be justified by new pharmacological studies highlighting the chemical nature of this plant, its effects on various parameters, and the mechanisms of the detected biological activities.
Financial support and sponsorship
This research was supported by Kashan University of Medical Sciences, Kashan, Iran (Grant No: 97001).
Conflicts of interest
There are no conflicts of interest.
| References|| |
Elliott TP, Kelley JM, Rawlin G, Spithill TW. High prevalence of fasciolosis and evaluation of drug efficacy against Fasciola hepatica
in dairy cattle in the Maffra and Bairnsdale districts of Gippsland, Victoria, Australia. Vet Parasitol 2015;209:117-24.
Mehmood K, Zhang H, Sabir AJ, Abbas RZ, Ijaz M, Durrani AZ, et al
. A review on epidemiology, global prevalence and economical losses of fasciolosis in ruminants. Microb Pathog 2017;109:253-62.
Mas-Coma S, Valero MA, Bargues MD. Chapter 2. Fasciola
, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Adv Parasitol 2009;69:41-146.
Martínez-Valladares M, Robles-Pérez D, Martínez-Pérez JM, Cordero-Pérez C, Famularo Mdel R, Fernández-Pato N, et al
. Prevalence of gastrointestinal nematodes and Fasciola hepatica
in sheep in the northwest of Spain: Relation to climatic conditions and/or man-made environmental modifications. Parasit Vectors 2013;6:282-90.
Gomez-Puerta LA, Gavidia C, Lopez-Urbina MT, Garcia HH, Gonzalez AE, Cysticercosis Working Group in Peru. Efficacy of a single oral dose of oxfendazole against Fasciola hepatica
in naturally infected sheep. Am J Trop Med Hyg 2012;86:486-8.
Ashrafi K, Valero MA, Panova M, Periago MV, Massoud J, Mas-Coma S. Phenotypic analysis of adults of Fasciola hepatica
, Fasciola gigantica
and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int 2006;55:249-60.
Moazeni M, Sharifiyazdi H, Izadpanah A. Characterization of Fasciola hepatica
genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1). Parasitol Res 2012;110:2379-84.
Moazeni M, Ahmadi A. Controversial aspects of the life cycle of Fasciola hepatica
. Exp Parasitol 2016;169:81-9.
Ullah R, Rehman A, Zafeer MF, Rehman L, Khan YA, Khan MA, et al
. Anthelmintic potential of thymoquinone and curcumin on Fasciola gigantica
. PLoS One 2017;12:e0171267.
Yamson EC, Tubalinal GA, Viloria VV, Mingala CN. Anthelmintic effect of betel nut ( Areca catechu
) and neem ( Azadirachta indica
) extract against liver fluke ( Fasciola
spp.). J Adv Vet Anim Res 2019;6:44-9.
Kelley JM, Elliott TP, Beddoe T, Anderson G, Skuce P, Spithill TW. Current threat of triclabendazole resistance in Fasciola hepatica
. Trends Parasitol 2016;32:458-69.
Tariq KA, Chishti MZ, Ahmad F, Shawl AS. Anthelmintic activity of extracts of Artemisia absinthium
against ovine nematodes. Vet Parasitol 2009;160:83-8.
Jeyathilakan N, Murali K, Anandaraj A, Abdul Basith S. In vitro
evaluation of anthelmintic property of ethno-veterinary plant extracts against the liver fluke Fasciola gigantica
. J Parasit Dis 2012;36:26-30.
Alvarez-Mercado JM, Ibarra-Velarde F, Alonso-Díaz MÁ, Vera-Montenegro Y, Avila-Acevedo JG, García-Bores AM. In vitro
antihelmintic effect of fifteen tropical plant extracts on excysted flukes of Fasciola hepatica
. BMC Vet Res 2015;11:45.
Nwofor SC, Morenikeji OA, Adeoye-Isijola O, Morenike AO, Oyetunde T, Oyeyemi OT. Inhibitory activities of ethanolic extracts of two macrofungi against eggs and miracidia of Fasciola
spp. Open Life Sci 2018;13:504-10.
Imtiyaz S, Rahman K, Sultana A, Tariq M, Chaudhary SS. Zingiber officinale
Rosc.: A traditional herb with medicinal properties. TANG [HUMANITAS MEDICINE] 2013;3:e26
Lin RJ, Chen CY, Chung LY, Yen CM. Larvicidal activities of ginger ( Zingiber officinale
) against Angiostrongylus cantonensis
. Acta Trop 2010;115:69-76.
Bazh EK, El-Bahy NM. In vitro
and in vivo
screening of anthelmintic activity of ginger and curcumin on Ascaridia galli
. Parasitol Res 2013;112:3679-86.
Lin RJ, Chen CY, Lu CM, Ma YH, Chung LY, Wang JJ, et al
. Anthelmintic constituents from ginger ( Zingiber officinale
) against Hymenolepis nana
. Acta Trop 2014;140:50-60.
Almalki E, Al-Shaebi EM, Al-Quarishy S, El-Matbouli M, Abdel-Baki AS. In vitro
effectiveness of Curcuma longa
and Zingiber officinale
extracts on Echinococcus
protoscoleces. Saudi J Biol Sci 2017;24:90-4.
Houshmand E, Kamalifar HS, Elmi H. In vitro
scolicidal effect of ginger ( Zingiber officinale
Roscoe) ethanolic extract against protoscolices of hydatid cyst. Iran. J Vet Med 2019;13:87-99.
Hassan FA, Abed GH, Abdel-Samii MA, El-Din MO. Antischistosomal activity of ginger aqueous extract against experimental Schistosoma mansoni
infection in mice. Biomark J 2016;2:1-6.
Mostafa OM, Eid RA, Adly MA. Antischistosomal activity of ginger ( Zingiber officinale
) against Schistosoma mansoni
harbored in C57 mice. Parasitol Res 2011;109:395-403.
Mostafa El-Sayed N, Mostafa El-Saka M. Anti-parasitic activity of Zingiber officinale
(Ginger): A brief review. Aperito J Bacteriol Virol Parasitol 2015;2:2-7.
Pereira CA, Oliveira LL, Coaglio AL, Santos FS, Cezar RS, Mendes T, et al
. Anti-helminthic activity of Momordica charantia
L. against Fasciola hepatica
eggs after twelve days of incubation in vitro
. Vet Parasitol 2016;228:160-6.
Shokri Mashhadi N, Ghiasvand R, Askari G, Hariri M, Darvishi L, Mofid MR. Anti-oxidative and anti-inflammatory effects of ginger in health and physical activity: Review of current evidence. Int J Prev Med 2013;4 Suppl 1:S36-42.
Yakhchali M, Bahramnejad K. Inhibition effect of pH on the hatchability of Fasciola
miracidia under laboratory conditions. Iran J Parasitol 2016;11:30-4.
Ashraf K, Halim H, Lim SM, Ramasamy K, Sultan S. In vitro
antioxidant, antimicrobial and antiproliferative studies of four different extracts of Orthosiphon stamineus
, Gynura procumbens
and Ficus deltoidea
. Saudi J Biol Sci 2020;27:417-32.
Abdullahi H, Karunakaran R, Sankar AU, Aye KM. Anti-inflammatory effect of Zingiber officinale
on Sprague Dawley rats. Asian J Pharm Clin Res 2017:10:353-5.
Moazeni M, Khademolhoseini AA. Ovicidal effect of the methanolic extract of ginger ( Zingiber officinale
) on Fasciola hepatica
eggs: An in vitro
study. J Parasit Dis 2016;40:662-6.
Arafa WM, Shokeir KM, Khateib AM. Comparing an in vivo
egg reduction test and in vitro
egg hatching assay for different anthelmintics against Fasciola
species, in cattle. Vet Parasitol 2015;214:152-8.
Hegazi AG, Megeed KN, Hassan SE, Abdelaziz MM, Toaleb NI, Shanawany EE, et al
. Comparative ovicidal activity of Moringa oleifera
leaf extracts on Fasciola gigantica
eggs. Vet World 2018;11:215-20.
Oyeyemi O, Adegbeyeni O, Oyeyemi I, Meena J, Panda A. In vitro
ovicidal activity of poly lactic acid curcumin-nisin co-entrapped nanoparticle against Fasciola
spp. eggs and its reproductive toxicity. J Basic Clin Physiol Pharmacol 2018;29:73-9.
Moxon JV, LaCourse EJ, Wright HA, Perally S, Prescott MC, Gillard JL, et al
. Proteomic analysis of embryonic Fasciola hepatica
: Characterization and antigenic potential of a developmentally regulated heat shock protein. Vet Parasitol 2010;169:62-75.
Kumarasingha R, Preston S, Yeo TC, Lim DS, Tu CL, Palombo EA, et al
. Anthelmintic activity of selected ethno-medicinal plant extracts on parasitic stages of Haemonchus contortus. Parasit Vectors 2016;9:187.
[Table 1], [Table 2]