|Year : 2017 | Volume
| Issue : 1 | Page : 13-16
Antibacterial activity of different extracts of prawn shell (Macrobrachium nipponense) against human bacterial pathogens
Katayoon Karimzadeh, Masoumeh Pormehr
Department of Marine Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran
|Date of Web Publication||26-Sep-2017|
Department of Marine Biology, Lahijan Branch, Islamic Azad University, Lahijan
Source of Support: None, Conflict of Interest: None
Aims: Bioactive compounds existing in crustacean shells have the potential to inhibit the growth of some pathogenic microorganism. The purpose of this study is to evaluate the antibacterial effects of different extracts of prawn shells (Macrobrachium nipponense) on some human pathogenic bacteria. Materials and Methods: Sampling (prawn) was conducted in summer 2014 from Anzali wetland in southern coast of Caspian Sea. Then, the hydroalcoholic, methanolic, and acetone extracts of prawn shells were applied for this purpose. Two Gram-positive (Bacillus subtilis Staphylococcus aureus) and three Gram-negative (Klebsiella pneumoniae, Vibrio cholerae, and Escherichia coli) were used as test organisms. The antibacterial activity was determined by paper disk diffusion. Results: The prawn shell extracts showed activity against pathogenic bacteria. The highest antibacterial activities were measured in B. subtilis, S. aureus, and V. cholerae with the zone of inhibition being 12.12 ± 0.32 mm, 12.51 ± 0.14 mm, and 12.35 ± 0.27 mm, respectively. Among all the strains, S. aureus exhibits a significant zone of inhibition against all extracts (P < 0.05). Conclusion: The findings of this research showed that different prawn shell extracts, particularly hydroalcoholic, have bactericidal effect on B. subtilis, S. aureus, and V. cholerae species.
Keywords: Antibacterial activity, pathogenic bacteria, prawn shell extracts
|How to cite this article:|
Karimzadeh K, Pormehr M. Antibacterial activity of different extracts of prawn shell (Macrobrachium nipponense) against human bacterial pathogens. Int Arch Health Sci 2017;4:13-6
|How to cite this URL:|
Karimzadeh K, Pormehr M. Antibacterial activity of different extracts of prawn shell (Macrobrachium nipponense) against human bacterial pathogens. Int Arch Health Sci [serial online] 2017 [cited 2021 Apr 21];4:13-6. Available from: http://www.iahs.kaums.ac.ir/text.asp?2017/4/1/13/215667
| Introduction|| |
The aquatic environment, which has a high biodiversity, is known as a huge resource for many natural products with medical, pharmaceutical, and food industry applications., Many natural products have been identified with potential pharmaceutical origins from aquatic invertebrates such as tunicates, sponges, corals, algae, and shells; they recognize that the capacity of antibiotic, antioxidant, and anticancer of these activities for these secondary metabolites among invertebrates.,, The crustacean shell has high potential of bioactive compounds with antimicrobial, antioxidant, and antitumor activities. The crustacean shell has a rich source of biological polymers, chitin (20%–30%), protein,and lipid pigments such as carotenoids and minerals. Chitin is the most important natural polymer which is converted to the nontoxic deacetylation form, chitosan (poly-b-1,4-2-amino-2-dixie-b-D-glu-kopiranoz). Recently, the role of extracted chitosan from different sources of aquatic animals in the fields of medicine, food, nutrition, and pharmaceuticals has received considerable attention.,
Some studies were carried out on antibacterial, antioxidant, and anticancer activities of crustacean shell extracts and chitosan from aquatic resources.,,,, These activate the defense system of a host and prevent the invasion of pathogenic microorganisms revealed by shell extracts., In recent years, human pathogenic microorganisms show antibiotic resistance to a wide range of antibiotics. Moreover, as synthetic drugs almost have some side effects, so finding alternative and secure resources with therapeutic potentials is necessary.,,
Aquatic natural compounds possess antimicrobial activity against various human pathogenic microorganisms. Therefore, by considering the importance of those products, the purpose of this study is to evaluate the antibacterial effects of different prawn shell extractions on common pathogenic bacteria.
| Materials and Methods|| |
Collection of prawn shell (Macrobrachium nipponense)
Prawns were collected in summer 2014 from Anzali wetlands in southern coast of Caspian Sea and transferred to tanks containing sea water with aerator in the biology laboratory (Lahijan Branch, Islamic Azad University). To complete anesthesia, temperature was set to zero degree. Afterward, the shells were removed from whole bodies and washed with distilled water. The removed shells were freeze-dried and grind to dried powder.
Preparation of extracts of prawn shell (Macrobrachium nipponense)
To prepare different extractions, 5 g of prawn powder dissolved in 150 ml of different solvents (75% of hydroalcoholic, acetone, and methanol) and were incubated for 48 h in a dark place. Then, they were filtered through filter paper., The solvents were evaporated by the lyophilizator and the obtained powders were weighed. Next, the consideration dose (2 mg/ml) of each extract was prepared using saline to resulting suspension.
Preparation of the bacteria test microorganisms
Staphylococcus aureus (ATCC 25923), Bacillus subtilis (ATCC 465), Escherichia More Details coli (ATCC 25922), Klebsiella pneumoniae (ATCC 10031), and Vibrio cholerae (ATCC 14035) provided by the Iranian Research Organization for Science and Technology were used as the bacterial tested organisms. Then, bacterial suspensions based on standard 0.5 McFarland (1.5 × 108 CFU/ml) were prepared.
In vitro antibacterial activities of different extracts of prawn shells were evaluated against five microbial stains using the well-agar diffusion method., Stock cultures were added to Muller-Hinton broth on the day before experiment and incubated for 24 h at 37°C. Different cultures of pathogenic bacteria were swabbed on the Muller-Hinton agar plates. Further, the filter paper discs (6 mm diameter) were impregnated with exact amounts of each extract. Standard antibiotic disks Gentamicin 10 and Erythromycin (Iran Daru Company) were used as the positive control.
The plates were incubated at 37°C for 24 h. Afterward, the inhibition zones formed on the media were measured. The positive antimicrobial activities were recorded based on the growth inhibition zone. All inhibition assays and controls were carried out in triplicate.
All data were expressed as mean ± standard deviation (SD), and statistical analyses were performed with the SPSS 17 software package (SPSS Inc., Chicago, USA). The significance of the results was tested by an analysis of variance and Duncan's multiple range test. The significance of differences was defined at P < 0.05.
| Results|| |
The antibacterial sensitivity results of three different extracts of prawn shells against the five different bacterial strains are shown in [Figure 1],[Figure 2],[Figure 3],[Figure 4],[Figure 5].
|Figure 1: Mean of inhibitory zone (mm) of different extractions dilutions of prawn shell against Staphylococcus aureus. Different letters indicate significant differences (P < 0.05)|
Click here to view
|Figure 2: Mean of inhibitory zone (mm) of different extractions dilutions of prawn shell against Escherichia coli. Different letters indicate significant differences (P < 0.05)|
Click here to view
|Figure 3: Mean of inhibitory zone (mm) of different extractions dilutions of prawn shell against Klebsiella pneumoniae. Different letters indicate significant differences (P < 0.05)|
Click here to view
|Figure 4: Mean of inhibitory zone (mm) of different extractions dilutions of prawn shell against Bacillus subtilis. Different letters indicate significant differences (P < 0.05)|
Click here to view
|Figure 5: Mean of inhibitory zone (mm) of different extractions dilutions of prawn shell against Vibrio cholerae. Different letters indicate significant differences (P < 0.05)|
Click here to view
The greatest action among different extracts was observed in V. cholerae and S. aureus. The hydroalcoholic extract was shown for the highest antibacterial activity against S. aureus than the other extracts [Figure 1], and all the extracts revealed significant action more than erythromycin (P < 0.05).
The maximum zone of inhibition (8.11 ± 0.21 mm) was exhibited against Ecoli in 1000 μg of the hydroalcoholic extract [Figure 2]. There was no antibacterial effect in the methanolic extract. There was a significant difference between different extracts against E. coli.
In K. pneumoniae, the greatest antibacterial effect appeared in hydroalcoholic and acetone extracts with 100 mg/ml of concentration. The methanolic extract did not show any antibacterial effect. The effects of hydroalcoholic and acetone extracts against K. pneumoniae were less than those of the gentamicin antibiotic and more than erythromycin [Figure 3].
The highest antibacterial effect in B. subtilis was related to hydroalcoholic and acetone extracts having zone of inhibition 11.25 ± 0.18 mm (at dose 500) and 12.12 ± 0.32 mm (at dose 1000) in hydroalcoholic and acetone, respectively [Figure 4]. Different extracts significantly showed various antibacterial effects against B. subtilis (P < 0.05).
The significant activity of the hydroalcoholic extract was observed against V. cholerae having zone of inhibition 12.35 ± 0.27 mm at a dose of 1000 mg/ml, while the metanolic extract did not show any antibacterial effect [Figure 5]. Different extracts exhibit significant differences against V. cholerae (P < 0.05).
| Discussion|| |
Recently, there was more attention to natural products and their application in pharmaceutical. The crustacean has been considered as a good source for bioactive substances among invertebrates.,
The results of the present study indicated that prawn shell extracts are more active against Gram-positive bacteria than Gram-negative bacteria.
The different extracts from Macrobrachium nipponense exhibited antibacterial activity against pathogenic bacteria as depicted in [Figure 1],[Figure 2],[Figure 3],[Figure 4],[Figure 5]. Among these strains, B. subtilis, S. aureus, and V. cholerae show the maximum zone of inhibition (12.12 mm, 12.51 mm, and 12.35 mm), respectively. Our results are comparable with the extracted chitosan from some crustacean shells.,,,, The zone of inhibition for the bactericidal effect of the ethanolic extract of crab shell against S. aureus and E. coli was reported about 13 mm and 10 mm at a concentration of 1000 μg/ml, respectively., However, there was no activity of crab shell (Portunus sanguinolentus) extract found against S. aureus., Liu demonstrated that antibacterial action of extracted chitosan in both Gram-negative bacteria and Gram-positive were effective, But it was more effective against gram-negative bacteria than Gram-positive. The antibacterial effects of chitosan and shell extracts on Gram-positive and Gram-negative bacteria are rather contradictory.,,, some studies reported that chitosan has more powerful effects on Gram-positive bacteria rather than on Gram-negatives, while others believe that high hydrophilic, which is observed in negative gram bacteria, make them more permeable than Gram-positives.,, It could be suggested that antimicrobial sensitively to chitosan can be related to the type of pathogenic organism and molecular weight of this polysaccharide.,
Hydrophilic solvents such as hydroalcoholic and acetone were more effective than nonpolar solvents (methanol), and the highest bacterial sensitivity was observed in the hydroalcoholic extract. Therefore, the better solubility of the active substances may be better soluble in polar solvents.,, Two mechanisms are probably involved in the antibacterial activity of prawn shells. First, the interaction of amino acid groups of prawn shells with cell surface components of bacteria, thus inhibiting their growth; and second, blocking the DNA replication by absorbing chitosan occurred.,,
It seems that in the present study, the greatest antibacterial effects of hydroalcoholic and acetone solvents, rather than methanol, on Gram-negative bacteria can be due to the solvents' polarity. As a chitosan has polycationic structure (pH <7), it destructs the bacteria membrane. The proton releasing from the amine group of shell can interact with the negative charge of the cell surface. This leads to the destruction of the bacterial cell and determines the potential of antibacterial activity.,,
| Conclusions|| |
The type of solvent can be involved in antibacterial effects in such a way that the hydroalcoholic extract of prawn shells is the most active extract against Gram-positive bacteria and even Gram-negative bacteria. The bactericidal of prawn shell extracts against pathogenic bacteria can be considered as an alternative therapeutic agent.
We are indebted to the research Vice Chancellor of Islamic Azad University Lahijan Branch, for supporting this research.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Chellaram C, Gnanambal KM, Edward JK. Antibacterial activity of the winged oyster Pteria chinensis
(Pterioida: Pteridae). Indian J Mar Sci 2004;33:369-72.
Proksch P, Edrada RA, Ebel R. Drugs from the seas-Current status and microbiological implications. Appl Microbiol Biotechnol 2002;59:125-34.
Takahashia T, Imaia M, Suzukia I, Sawai J. Growth inhibitory effect on bacteria of chitosan membranes regulated by the deacetylation degree. Biochem Eng J 2008;40:485-91.
Haug T, Kjuul AK, Styrvold OB, Sandsdalen E, Olsen ØM, Stensvåg K. Antibacterial activity in Strongylocentrotus droebachiensis
), Cucumaria frondosa
), and Asterias rubens
). J Invertebr Pathol 2002;81:94-102.
Shahidi F, Synwieki J. Isolation and characterization of nutrients and value added products from snow crab (Chionoecetes opilio
) and shrimp (Pandalus borealis
) processing discards. J Agric Food Chem 1991;39:1532-72.
Yilmaz E. Chitosan: A versatile biomaterial. Adv Exp Med Biol 2004;553:59-68.
Brine P, Sandford A, ZikakisJP, editors. Advnces in chitin and chitosan. Proceedings from the Fifth International Conference on Chitin and Chitosan. London: Elsevier; 1992. p. 472-8.
Li B, Wang X, Chen R, Huangfu V, Xie G. Antibacterial activity of chitosan solution against Xanthomonas
pathogenic bacteria isolated from Euphorbia pulcherrima
. Carbohydr Polym 2008;72:287-92.
Jeon YJ, Park PJ, Kim SK. Antimicrobial effect of chito oligosaccharides produced by bioreactor. Carbohydr Polym 2001;44:71-6.
Kong M, Chen XG, Xue YP, Liu CS, Yu LG, Ji QX, et al
. Preparation and antibacterial activity of chitosan microspheres in a solid dispersing system. Front Mater Sci China 2008;2:214-20.
Huang X, Huang X, Jiang XH, Hu FQ, Du YZ, Zhu QF, et al
. In vitro
antitumour activity of stearic acid-g-chitosan oligosaccharide polymeric micelles loading podophyllotoxin. J Microencapsul 2012;29:1-8.
Maeda Y, Kimura Y. Antitumor effects of various low-molecular-weight chitosans are due to increased natural killer activity of intestinal intraepithelial lymphocytes in sarcoma 180-bearing mice. J Nutr 2004;134:945-50.
Park BK, Kim MM. Applications of chitin and its derivatives in biological medicine. Int J Mol Sci 2010;11:5152-64.
Younes I, Rinaudo M. Chitin and chitosan preparation from marine sources. Structure, properties and applications. Mar Drugs 2015;13:1133-74.
Gokilavani S, Vijayabharathi V, Parthasarathy R. Physico-chemical characteristics and antibacterial activity of chitosan extracted from shell of crab Paratelphusa hydrodromous
. Asian J Res Pharma Sci 2014;4:125-8.
Nisha G. Antibacterial effect of crab shell extract against human pathogenic bacteria. Int J Sci Eng Tech Res 2015;5790-1.
Lennette EH. Manual of Clinical Microbiology. 4th
ed. Washington, DC: American Association for Microbiology; 1985.
Zhong Z, Xing R, Liu S, Wang L, Cai S, Li P. Synthesis of acyl thiourea derivatives of chitosan and their antimicrobial activities in vitro
. Carbohydr Res 2008;343:566-70.
Sagoo SK, Board R, Roller S. Chitosan potentiates the antimicrobial action of sodium benzoate on spoilage yeasts. Lett Appl Microbiol 2002;34:168-72.
Liu H, Du Y, Yang J, Zhu H. Structural characterization and antimicrobial activity of chitosan/betaine derivative complex. Carbohydr Polym 2004;55:291-7.
Rabea EI, Badawy ME, Stevens CV, Smagghe G, Steurbaut V. Chitosan as antimicrobial agent: Applications and mode of action. Biomacromolecules 2003;4:1457-65.
Wei D, Sun W, Qian W, Ye Y, Ma X. The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydr Res 2009;344:2375-82.
Feng Y, Xia W. Preparation, characterization and antibacterial activity of water-soluble O-fumaryl-chitosan. Carbohydr Polym 2011;83:1169-73.
Vilar Junior JC, Ribeaux DR, Alves da Silva CA, De Campos-Takaki GM. Physicochemical and antibacterial properties of chitosan extracted from waste shrimp shells. Int J Microbiol 2016;2016:1-7. Article ID 5127515.
Helander IM, Wright AV, Mattila-Sandholm TM. Potential of lactic acid bacteria and novel antimicrobials against gram-negative bacteria. Trends Food Sci Technol 1997;8:146-50.
No HK, Park NY, Lee SH, Meyers SP. Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int J Food Microbiol 2002;74:65-72.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]