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IAHS 2016, 3(3): 125-129 Back to browse issues page
Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital
F. Nourbakhsh *1, H. Momtaz 2
1- Department of Pharmacodynamy & Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran , fahimeh_nourbakhsh@yahoo.com
2- Department of Microbiology, Faculty of Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract:   (514 Views)

Abstract

Aims: The increasing usage of antibiotics can cause resistance to the treatment of infections, which can caused by bacteria, e.g. Pseudomonas aeruginosa. The aim of this study was to trace the class I, II and III integrons in isolates of P. aeruginosa of nosocomial infection and determining the antibiotic resistance pattern of the bacteria.

Instrument & Methods: In this cross-sectional study, 100 Pseudomonas aeruginosa clinical isolates of infected wounds, bedsores, burns, urinary tract infections and respiratory tract infections were collected from patients of 3 Isfahan City hospitals, Iran (Al Zahra, Kashani, Shariati) in 2015.  After identification tests and antibiogram, integrons class I, II and III were detected by M-PCR method. Data analysis were performed in SPSS 16 software using Chi-square and Fisher exact tests and the relationship between the presence of class III, II, I was calculated by M-PCR test.

Findings: All isolates had multiple antibiotic resistances. The highest antibiotic resistance was to Tetracycline (85%) and the lowest to Norfloxacin (12.5%). There were significant differences between class I and the two other classes of integrons (p=0.036). There was a statistically significant difference between the presence of blaTEM gene in Pseudomonas aeruginosa with other coding genes for antibiotic resistance (p=0.029).

Conclusion: Pseudomonas aeruginosa isolates are multi-drug resistant and almost all isolates from clinical infections have class I, II and III Integrons.

Keywords: Pseudomonas aeruginosa, Integrons, Multiple Antibiotic Resistance, Hospital Infections
Full-Text [PDF 401 kb]   (358 Downloads)    
Article Type: Original Research | Subject: Epidemiology, Clinical Aspects of Tropical Diseases Caused by Virus, Bacteria, Algae, Fungi and Parasites
Received: 2016/05/18 | Accepted: 2016/07/13 | Published: 2016/11/15
* Corresponding Author Address: Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Shahid Fakouri Street, Mashhad, Iran. Postal Code: 9177899191
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Nourbakhsh F, Momtaz H. Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital. IAHS. 2016; 3 (3) :125-129
URL: http://iahs.kaums.ac.ir/article-1-88-en.html


issue 3, Issue 3 (Summer 2016) Back to browse issues page
International Archives of Health Sciences
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