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IAHS 2015, 2(4): 139-143 Back to browse issues page
Antibacterial Activity of the Hydro-Alcoholic Extract of Juglans regia L. Stem Bark on Human Bacterial Infection
N. Moori Bakhtiari *1, E. Khalafi2
1- Microbiology Department, Veterinary Medicine Faculty, Shahid Chamran University, Ahvaz, Iran , n.moori@scu.ac.ir
2- Veterinary Medicine Faculty, Shahid Chamran University, Ahvaz, Iran, Ahvaz, Iran
Abstract:   (634 Views)

Abstract

Aims: Bovine mastitis continues to be the most costly disease to the dairy farmers. It dominates in Iran as one of the most prevalent diseases in dairy cattle among the dairy farms. Mastitis treatment with antibiotics leads to the development of antibiotic resistant strains and consumer health problem. This study was performed for the first time to analyze in vitro effects of hydro-alcoholic extract of Juglans regia L. stem bark on 6 mastitis pathogens.

Materials & Methods: the susceptibility of 6 strains of bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus spp., Pasteurella multocida and Mannheimia haemolytica) were analyzed against hydro-alcoholic extract of Juglans regia L. stem bark with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods.

Findings: Hydro-alcoholic extract did not have antibacterial effects on E. coli and K.  pneumoniae. Minimum inhibitory concentration for S. aureus, P. multocida, M. haemolytica and Streptococcus spp. was 62.5mg/ml of hydro-alcoholic extract. There was not any significant response with concentrations below 100mg/disc on S. aureus, Streptococcus species, P. multocida and M. haemolytica. Minimum bactericidal concentration of this extract was 100mg/ml in all isolates.

Conclusion: Juglans regia L. have some antibacterial effects on S. aureus, P. multocida, M. haemolytica and Streptococcus species.

Keywords: Mastitis, Bovine, Disk Diffusion Antimicrobial Tests, Juglans
Full-Text [PDF 399 kb]   (900 Downloads)    
Article Type: Original Research | Subject: Epidemiology, Clinical Aspects of Tropical Diseases Caused by Virus, Bacteria, Algae, Fungi and Parasites
Received: 2015/09/8 | Accepted: 2015/11/29 | Published: 2015/12/7
* Corresponding Author Address: Department of pathobiology, Veterinary Medicine Faculty, Shahid Chamran University, Golestan Street, Ahvaz, Iran
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Moori Bakhtiari N, Khalafi E. Antibacterial Activity of the Hydro-Alcoholic Extract of Juglans regia L. Stem Bark on Human Bacterial Infection . IAHS. 2015; 2 (4) :139-143
URL: http://iahs.kaums.ac.ir/article-1-65-en.html
Volume 2, Number 4 (Fall 2015) Back to browse issues page
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